Dissertation / PhD Thesis/Book

PreJuSER-33320

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Reaktionstechnische Untersuchungen zur sekretorischen Produktion von rekombinantem, humanem Chymotrypsinogen B mit Pichia pastoris

Curvers, S. (Corresponding author)FZJ*

2001
Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag Jülich

Please use a persistent id in citations: http://hdl.handle.net/2128/19973

Report No.: Juel-3867

Abstract: This study describes the development of fermentation technology for the secretory production of recombinant, human chymotrypsinogen B using the methylotrophic yeast Pichia pastoris in an integrated approach of genetic engineering, fermentation and downstream processing. Three different fermentation approaches have been developed, each respecting the demands of an industrial production process in a different way. In a fed-batch process using methanol as the sole source of carbon and energy during the production, a space-time-yield of 5.4 mg$\cdot$1$^{-1}$ $\cdot$h$^{-1}$, combined with a high product titer of 480 mg$\cdot$1$^{-1}$ and biomass concentration of 150 g$\cdot$1$^{-1}$ cdw has been achieved. Avoiding the high oxygen demand of the latter high-cell-density process, two different continuous approaches have been investigated on, yielding space-time-yields of 25 mg$\cdot$1$^{-1}$ $\cdot$h$^{-1}$, using methanol as the sole substrate and 10.3 mg$\cdot$1$^{-1}$ $\cdot$h$^{-1}$ in a simplified mixed-feed approach using glucose and methanol, respectively.

Note: Record converted from VDB: 12.11.2012
Note: Aachen, Techn. Hochsch., Diss., 2001

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Contributing Institute(s):

1. Biotechnologie 2 (IBT-2)

Research Program(s):

1. Verfahrenstechnik zur mikrobiellen Gewinnung von Primärmetaboliten (41.40.0)

Appears in the scientific report 2001

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Database coverage:

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